| DNA may be small and it may seem complicated but | | | | paternity test extraction processes may be |
| the mystery behind deoxyribonucleic acid is unfolding | | | | somewhat different than the process experiment |
| quickly (even as you read this article most likely). An | | | | that I am about to introduce to you but many of the |
| increased understanding of DNA also continues to | | | | principles are still the same. |
| generate ideas for real-life applications, many of | | | | Some online content2 sponsored by the University of |
| which are fascinating forms of DNA testing. | | | | Utah shows you how you can extract the DNA from |
| How can something so small and composed of | | | | dried peas. The great thing is that you can try this |
| seemingly obscure as deoxyribonucleic acid play a | | | | from home. You can take a look at the content for |
| part in our lives? One answer is the paternity test. | | | | more info but I will show you a quick paraphrased |
| The Paternity Test: One Application for DNA | | | | process that can be followed as well: |
| A paternity test is accomplished by taking a sampling | | | | 1) Open the Cell |
| of a child's DNA and a parent's DNA to exclude or | | | | The online content says you can do this with some |
| include the possibility of parentage. Testing the DNA | | | | water and a blender! |
| of a confirmed parent and a potential parent can help | | | | Simply buy a bag of dried peas and blend them with |
| lab scientists determine paternity more quickly but | | | | water. |
| the DNA of two parents is not required. Lab | | | | 2) Open the Cell's Nucleus |
| scientists can often prove paternity by exclusion | | | | The online content suggests that you use detergent |
| (100% accuracy) or by inclusion (99.9% accuracy). | | | | (of the liquid variety) |
| A paternity test may seem complicated but now | | | | Open the cell's nucleus. Simply mix the detergent with |
| that it's down to a science it's not quite the mystery | | | | the pea mixture. |
| that it seems to have been in the past. I found a | | | | 3) Separate the DNA from its Protective and |
| brief online explanation1 which led me to additional | | | | Architectural Proteins |
| questions, additional answers and the rest of this | | | | The online content suggests that you use a |
| article! | | | | tenderizer of meats or thejuice from a pineapple to |
| 1) The Sampling | | | | separate the proteins from the DNA sample. |
| First of all, there must be a sampling of DNA taken | | | | 4) Separate the Floating Fats and Proteins from the |
| from a living specimen like you or me. However you | | | | Floating DNA |
| can't just reach in and grab a chunk of DNA like you | | | | The online content suggests doing this by adding |
| would dig into a cracker barrel. Nope-it's a bit more | | | | alcohol to the pea liquid |
| complicated. The first step of DNA extraction is to | | | | Do not mix; the alcohol should create a top layer of |
| take a sample of blood or saliva. (You can easily get | | | | liquid over the pealiquid. |
| a sample of saliva by swabbing the inside of your | | | | 5) Take a Look at Your DNA sample |
| cheek). This "swab sample" will provide a lab | | | | Since the DNA will choose "to dwell" in the alcohol |
| professional with a DNA sample that is still mixed with | | | | and the fats andproteins will stay in the water, the |
| other molecules and chemical make-ups that you | | | | DNA should finally be visible at thispoint. |
| wouldn't need for the paternity test. So, you have to | | | | Of course, with human cells, the process might not |
| separate the DNA from those other substances. It's | | | | be so simple, but I think the example mentioned |
| like separating the crackers from several other | | | | above provides a good visualization of a process that |
| samples that are in the same barrel. | | | | is probably very similar. |
| 2) The Extraction | | | | To learn more about the next steps in analyzing DNA |
| Okay. So how do the lab scientists extract DNA | | | | and to learn more about how DNA might be used in |
| from the rest of the substances floating around? | | | | a paternity test, please read part II of "DNA |
| This is where the fun begins and you don't even | | | | Applications and the Paternity Test." (To locate the |
| have to be a lab professional to try your own | | | | article simply "Google" the article title along with my |
| extraction process. Keep in mind however that the | | | | (the author's) name). |